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Steam Sterilization

Sterilization usually refers to the complete killing of all living organisms, including spores. Spores, as the name implies, some bacteria produce special reproductive cells called spores, for example, when an infected mosquito bites a human, spores are injected into the blood. and fungi spread by means of spores. Common sterilization techniques include four types, they are the application of wet heat, dry heat, chemicals and radiation. It is also important to note that the type of material, container, and the quantity of items to be sterilized determines which method to use. In the laboratory animal facilities, various pieces of equipment are used for sterilization, but the autoclave is the primary means of sterilizing supplies. For the animals laboratory, the sterilization is of vital importance, because the laboratory animals are easy to be infected with the bacteria.
Despite built-in good safeguards, an autoclave presents the possibility of serious injury to laboratory technicians from hot surface and from the release of live steam. It is important, therefore, that laboratory animal technicians understand the proper operation, the limitations, and the safeguards for sterilization by autoclave. Even though the manual is able to aid the laboratory animal technicians in understanding the equipment, so it is very necessary for the laboratory animals technicians to have firm understanding and experienced in instrumentation engineering and design.


Pure Steam Sterilizer

It is the responsibility of the facility management manager to ensure that all autoclave equipment is appropriately monitored, maintained in good working order, and available for research personal as requested. For technology firms, the high quality research fellows have become key resources. Meanwhile the facility management manager is responsible for ensuring all research and technical staff are adequately trained and experienced in autoclave sterilization procedures. For example, there should be regular leak tests on the chamber when a vacuum phase is part of the cycle. Sterilization records should be available for each sterilization run, and they should be approved ad part of the batch release procedure.
Autoclave sterilization times and temperature are dependent on the composition and nature of the items which need to be sterilized. Minimum times are usually 15 minutes at 121 0C or 5 minutes at 132.2 0C. It adopts temperature time and F 0 valve double-monitor to ensure the sterilizing results. Small items to be sterilized should be bagged or wrapped in heat-resistant plastic, paper, or cloth. It is worth mentioning that when sterilizing items using pressure resistant bags, living the bag open during the whole sterilization process will facilitate steam penetrating the contents of the bag. When sterilizing bio-hazardous waste, bags or containers which use to pack waste should not be overfilled, half to three quarters full is recommended. Add water to the bag or container before sealing the container, this act will able to aid in the generation of steam within the container during the sterilization process. Do not add water if there is a chance that the materials which need to be sterilized may splash out of the container. Remind again, when the autoclave cycle is complete and the pressure returns to zero, then the door may be opened.

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